Mycotoxins

Mycotoxins

The term “mycotoxin” refers to a family of toxic secondary metabolites produced by moulds. While hundreds of mycotoxins exist in nature, only a select few are relevant to the agro-food industry: aflatoxins, deoxynivalenol, fumonisins, ochratoxin, T-2 and HT-2 toxins and zearalenone.

 

When animals consume feed containing mycotoxins, they not only become ill, but it triggers a domino-effect across the whole livestock industry, whereby contaminated foods of animal origin, such as milk, pose a risk to human health, resulting in large economic losses. These compounds have reportedly been associated with various human and animal diseases and are unfortunately a well-established threat to grains and other commodities.

 

Mycotoxins concern not only grains, coffee, cocoa, wine and spices, but are also prevalent in the dairy industry, particularly aflatoxin M1. The on-site detection of patulin, a harmful mycotoxin found in fruits and fruit juices is extremely important for juice processors and infant food producers.

Mycotoxin ELISA Kits

We provide a comprehensive portfolio of ELISA test kits that suit all needs for reliable and effective mycotoxin analysis.

 

Multiple mycotoxin assays can also be run by our automatic analyzers and have been validated for The BOLTTM and ThunderBOLT®, two compact, user-friendly ELISA robots that can create considerable savings.

Aflatoxin Testing in milk with an AOAC PTM: I’screen AFLA M1 Milk ELISA Kit

The I’screen AFLA M1 Milk is a quantitative immunoassay for the analysis of aflatoxin M1. The kit has been granted AOAC Research Institute Performance Tested MethodsSM status (AOAC Certificate No. 072002) for use with raw bovine whole milk, skim milk and powdered milk.

The limit of detection (LOD) is 5 ng/L for liquid milk and 50 ng/L for milk powder. Its sensitivity allows the detection of aflatoxin M1 at concentrations below the regulatory limits set by the European Union. The assay is compliant with the ISO 14675:2003 (IDF 186:2003) guideline. I’screen AFLA M1 Milk Product Page

Mycotoxins in grains, feed and vegetal commodities: a comprehensive portfolio of ELISA kits lines

  • B ZERO®: ELISA test kits for low analytical volumes. No calibration curve means no hidden costs – with the same nr of wells as regular ELISA kits, the B ZERO is able to run more samples. Results obtained in less than 30 min. The kits can be processed manually or with The BOLTTM or the ThunderBOLT® automatic analyzer.
  • Celer®: fast ELISA kits for rapid screening. All Celer® assays are validated on The The BoltTM automated ELISA system.
  • I’Screen: accurate, precise, high sensitive ELISA test kits with low detection limits

Analytes

  • Aflatoxin M1
  • Total B and G aflatoxins
  • Aflatoxin B1 specific
  • Deoxynivalenol
  • Fumonisins
  • Ochratoxin A
  • Patulin
  • T-2 and HT-2 toxins
  • Zearalenone

Support and Training

We offer different ELISA trainings suited to the different needs and diverse levels of technical expertise of our partners. Customized sessions (basic or advanced level) are offered for laboratory personnel and managers, either in group or inidvidual sessions.

Control Materials, Standards and EIA reagents

To complete your mycotoxins screening, we provide solutions including matrix-based control materials and standard solutions. These products are relevant both in the evaluation and validation process and in routine Internal Quality Control and External Quality Assurance.

 

To support the routine execution, either manually or through automation, Gold Standard Diagnostics provide additional standards and specific solutions combined with our ELISA portfolio.

Immunoaffinity Columns

For the majority of regulated mycotoxins, we offer an extensive portfolio of mycotoxin immunoaffinity columns. Our SENSIColumn IACs are simple and reliable sample purification solutions.

Key Benefits of the IACs

  • Excellent flow behaviour
  • Highly specific purification with monoclonal antibodies
  • Availability of specific columns and “combi” columns for multi-mycotoxin groups
  • Wide range of applications for various matrices
  • Multi-analyte IACs for LC-MS/MS application
  • Long shelf-life
  • Fast (15-30 mins)
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