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Anabaenopeptins, ELISA, 96-test
Anabaenopeptins, ELISA, 96-test
SKU 520070 Category
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Product Details

Catalogue Number

– 520070

Category

– Water
– Anabaenopeptins, ELISA, 96-test

Brand

– GSD Abraxis
– Water

Features & Benefits

– The Anabaenopeptins ELISA is an immunoassay for the quantitative and sensitive detection of Anabaenopeptins in water samples. This test is suitable for the quantitative and/or qualitative detection of Anabaenopeptins in water samples. If necessary, positive samples can be confirmed by LC/MS, protein phosphatase assay, or other conventional methods. The test is a direct competitive ELISA for the detection of Anabaenopeptins. It is based on the recognition of Anabaenopeptins by specific antibodies. Toxin, when present in a sample, and an Anabaenopeptins-HRP analogue compete for the binding sites of the rabbit anti-Anabaenopeptins antibodies in solution. The anti-Anabaenopeptins antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and an addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anabaenopeptins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.

Details

– The Anabaenopeptins ELISA is an immunoassay for the quantitative and sensitive detection of Anabaenopeptins in water samples. This test is suitable for the quantitative and/or qualitative detection of Anabaenopeptins in water samples. If necessary, positive samples can be confirmed by LC/MS, protein phosphatase assay, or other conventional methods. The test is a direct competitive ELISA for the detection of Anabaenopeptins. It is based on the recognition of Anabaenopeptins by specific antibodies.
Toxin, when present in a sample, and an Anabaenopeptins-HRP analogue compete for the binding sites of the rabbit anti-Anabaenopeptins antibodies in solution. The anti-Anabaenopeptins antibodies are then bound by a second antibody (goat anti-rabbit) immobilized on the wells of the microtiter plate. After a washing step and an addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Anabaenopeptins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
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