B ZERO DON v2 (48 tests)
Assay for deoxynivalenol detection in cereals, feed and DDGSs.
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Product Details
Catalogue Number
– HU0040028
Category
– Mycotoxins
Method
– ELISA
– Assay for deoxynivalenol detection in cereals, feed and DDGSs.
Brand
– ET Hungary
– Rice ,wheat,Groundnut,Maize and Animal Feed manufacturers
– Cereals, feed, DDGS, wheat bran and middlings
Features & Benefits
– ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers). The assay is validated on The BoltTM automated ELISA system.
Details
– ELISA test kit for the quantitative detection of deoxynivalenol in cereals, feed and DDGS (Distillers).
Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.
Advantages: B ZERO® is the most cost-effective line of ELISA test kits. Thanks to the removal of the calibrators, the analysts are not required to use wells for the standard curve. Quantitative results are obtained by means of a virtual master-curve that is provided within each kit batch.
Test Principle
– Microplate competitive enzyme immunoassay without standard curve. Sample quantification through interpolation of inhibition value on virtual curve (master-curve) provided for each batch of kit.
Format
– Microtiter plate with 48 wells
Limit of Detection
– Wheat, Maize, Barley, Feed and DDGS – 0.04 ppm
Durum Wheat – 0.12 ppm
Wheat Bran and Middlings – 0.24 ppm
Durum Wheat – 0.12 ppm
Wheat Bran and Middlings – 0.24 ppm
Sample Preparation
– Cereals (Maize, Wheat), Feed, DDGS – grinding, methanol-water extraction, filtration
Durum Wheat – grinding, water extraction, centrifugation, dilution
Wheat Bran and Middlings – water extraction, activated charcoal clean-up, filtration, dilution
Durum Wheat – grinding, water extraction, centrifugation, dilution
Wheat Bran and Middlings – water extraction, activated charcoal clean-up, filtration, dilution
Specificity
– Deoxynivalenol 100%
3-acetyl-deoxynivalenol >100%
3-glucosyl-deoxynivalenol 64%
15-acetyl-deoxynivalenol 2%Nivalenol <4%
3-acetyl-deoxynivalenol >100%
3-glucosyl-deoxynivalenol 64%
15-acetyl-deoxynivalenol 2%Nivalenol <4%