– The Glyphosate ELISA Plate and Derivatization Kit is an immunoassay for the quantitative and sensitive screening of Glyphosate in water samples. This test is suitable for the quantitative and/or qualitative screening of Glyphosate in groundwater, surface water, well, and tap water samples.For soil, crop, and food sample applications, contact us for the appropriate technical bulletin and/or matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Glyphosate by polyclonal antibodies. The sample to be tested is derivatized and then added to microtiter wells coated with goat anti-rabbit antibodies. A rabbit anti-Glyphosate antibody solution is added to the wells with the derivatized samples and allowed to incubate for 30 minutes. The Glyphosate enzyme conjugate is then added and a competitive reaction occurs between the Glyphosate, which may be present in the sample, and the enzyme labeled Glyphosate for the binding sites of the rabbit anti-Glyphosate antibodies bound by the goat anti-rabbit antibodies immobilized on the microtiter plate. The reaction is allowed to continue for 60 minutes.
After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Glyphosate present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.