– Magnetic particle kit for detection and quantitation of glyphosate in water (groundwater, surface water, well water). For soil, crop, and food use contact the company for application bulletins and/or specific matrix validation guidelines. The Glyphosate Kit applies the principles of enzyme linked immunosorbent assay (ELISA) to the determination of glyphosate. The sample to be tested is derivatized and then added, along with paramagnetic particles attached with antibodies specific to glyphosate and incubated for 30 minutes. The glyphosate enzyme conjugate is then added, at this point a competitive reaction occurs between the glyphosate which may be be in the sample and the enzyme-labeled glyphosate analog for the antibody binding sites on the magnetic particles.
The reaction is allowed to continue for thirty (30) minutes. At the end of the incubation period, a magnetic field is applied to hold in the test tube the para-magnetic particles (with glyphosate and labeled glyphosate bound to the antibodies on the particles, in proportion to their original concentration), and allow the unbound reagents to be decanted. After decanting, the particles are washed with Washing Solution. The presence of glyphosate is detected by adding the Color Solution, which contains the enzyme substrate (hydrogen peroxide) and the chromogen (3,3′,5,5′-tetramethylbenzidine). The enzyme-labeled glyphosate bound to the glyphosate antibody catalyzes the conversion of the substrate/ chromogen mixture to a colored product. After an incubation period, the reaction is stopped and stabilized by the addition of a diluted acid (Stopping Solution). Since the labeled glyphosate (conjugate) was in competition with the unlabeled glyphosate (sample) for the antibody sites, the color developed is inversely proportional to the concentration of glyphosate in the sample.