– The Monensin ELISA is an immunoassay for the quantitative and sensitive screening of Monensin. This test is suitable for the quantitative and/or qualitative screening of Monensin in animal feed and contaminated products. For additional matrices, contact Eurofins Abraxis technical services for application bulletins and/or specific matrix validation guidelines. Samples requiring regulatory action should be confirmed by HPLC, GC/MS, or other conventional methods.
The test is a direct competitive ELISA based on the recognition of Monensin by specific antibodies. Monensin, when present in a sample, and a Monensin-HRP analogue compete for the binding sites of sheep anti-Monensin antibodies in solution. The Monensin antibodies are then bound by a second antibody (donkey anti-sheep) immobilized on the wells of the microtiter plate. After a washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Monensin present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.