DNAnimal Ident Meat IPC Kit for identification of bovine and porcine DNA (Copy)
2×48 RT-PCR rxns with IPC, all reagents and positive control DNA included. Validated for ABI7900, ABI7500/Fast, MX3005P/MX3000P, AriaMX, CFX96 and LC480.
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Product Details
Catalogue Number
– 5422212010-1
Category
– Animal Species
Method
– PCR
– 2×48 RT-PCR rxns with IPC, all reagents and positive control DNA included. Validated for ABI7900, ABI7500/Fast, MX3005P/MX3000P, AriaMX, CFX96 and LC480.
Brand
– ET Hungary
– Food testings labs/Meat Exporters/ Five star hotels/Meat Research labs
Kit Components
– (1x) OligoMix: OM QL RT IPC (LR/HR+)Module GS-P-08.030 Cattle
390 µL primer/probe mix for bovine DNA detection
(1x) OligoMix: OM QL RT IPC (LR/HR+) Module GS-P-08.041 Pig
390 µL primer/probe mix for bovine DNA detection
(2x) BasicMix: BM (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U
650 µL reaction mix without primers and probes
(2x) ROX™ dye, 23 µL
(1x) Genomic DNA Cattle [Bos taurus] 50 µL
(1x) Genomic DNA Pig [Sus scrofa domestica]50 µL
(2x) DNA stabilization buffer (for NTCs)
390 µL primer/probe mix for bovine DNA detection
(1x) OligoMix: OM QL RT IPC (LR/HR+) Module GS-P-08.041 Pig
390 µL primer/probe mix for bovine DNA detection
(2x) BasicMix: BM (NR) GS-P-26.012 EFGi TP 2x (NR) 1.5U
650 µL reaction mix without primers and probes
(2x) ROX™ dye, 23 µL
(1x) Genomic DNA Cattle [Bos taurus] 50 µL
(1x) Genomic DNA Pig [Sus scrofa domestica]50 µL
(2x) DNA stabilization buffer (for NTCs)
Material Required But Not Included
– water, DNase-freeVortexMicropipettes (variable 1-1000 µL) and filter tipsPCR optical tubes or plates 0.2 ml and optical caps or sealsRT-PCR Thermocycler
Features & Benefits
– Test kit for the qualitative detection of bovine and porcine DNA with 2 x 48 real-time PCR reactions
PCR Cycler platform
– Agilent MX3005P/3000P, Agilent AriaMX, ABI7500/Fast, Bio-Rad CFX Touch,Roche LC480 and ABI 7900HT
Limit of Detection
– LODabs ≤ 10 copies; LODrel ≤ 0.01%
Storage Instructions
– -20°C